Electrophoresis-assisted accumulation of conductive nanoparticles for the enhancement of cell electropermeabilization

The use of conductive nanoparticles (NPs) was previously proposed as a way to locally amplify the electric field (EF) intensity at cell membrane enhance electroporation. To achieve this, close distance between NPs and is mandatory. Here, new method improve contact surface using effects pulses (electrophoretic forces) explored. two types are analyzed alone or combined in two-pulse-train protocol on Chinese hamster DC-3F cells. Particularly we used 100 µs duration pulses, low intensity-millisecond combinations both. Finally, studied coated (PEGylated) for this application. Our results demonstrate that delivery an prior electroporation increases accumulation around suggesting pushed towards through electrophoretic forces. This allowed reducing need long incubations cells observe enhancement mediated by NPs. Thus can be increase either aggregated individual (i.e. PEGylated) supporting nature observed effects.